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normal human embryonic lung fibroblast hfl1  (ATCC)


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    ATCC normal human embryonic lung fibroblast hfl1
    MiR-425 is up-regulated and CPEB1 is down-regulated in lung cancer tissues; miR-425 targets CPEB1. A. RT-qPCR detection of miR-425 expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); B. RT-qPCR detection of CPEB1 mRNA expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); C-D. Protein bands and protein expression of CPEB1 by Western blot analysis (n = 93); E. Pearson correlation analysis of the relation between miR-425 expression and CPEB1 mRNA expression in lung cancer patients; F. miR-425 and CPEB1 mRNA expression in <t>HFL1</t> and lung cancer cell lines (A549, NCI-H1299 and NCI-H292); G. Targetscan prediction of the targeting relationship between miR-425 and CPEB1; H. Dual luciferase reporter gene assay validation of the targeting relationship between miR-425 and CPEB1. Data in the figure were expressed by mean ± standard deviation; Comparison between two groups was analyzed by t-test.
    Normal Human Embryonic Lung Fibroblast Hfl1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human embryonic lung fibroblast hfl1/product/ATCC
    Average 96 stars, based on 630 article reviews
    normal human embryonic lung fibroblast hfl1 - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "Human bone marrow mesenchymal stem cell-derived exosomes containing microRNA-425 promote migration, invasion and lung metastasis by down-regulating CPEB1"

    Article Title: Human bone marrow mesenchymal stem cell-derived exosomes containing microRNA-425 promote migration, invasion and lung metastasis by down-regulating CPEB1

    Journal: Regenerative Therapy

    doi: 10.1016/j.reth.2022.03.007

    MiR-425 is up-regulated and CPEB1 is down-regulated in lung cancer tissues; miR-425 targets CPEB1. A. RT-qPCR detection of miR-425 expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); B. RT-qPCR detection of CPEB1 mRNA expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); C-D. Protein bands and protein expression of CPEB1 by Western blot analysis (n = 93); E. Pearson correlation analysis of the relation between miR-425 expression and CPEB1 mRNA expression in lung cancer patients; F. miR-425 and CPEB1 mRNA expression in HFL1 and lung cancer cell lines (A549, NCI-H1299 and NCI-H292); G. Targetscan prediction of the targeting relationship between miR-425 and CPEB1; H. Dual luciferase reporter gene assay validation of the targeting relationship between miR-425 and CPEB1. Data in the figure were expressed by mean ± standard deviation; Comparison between two groups was analyzed by t-test.
    Figure Legend Snippet: MiR-425 is up-regulated and CPEB1 is down-regulated in lung cancer tissues; miR-425 targets CPEB1. A. RT-qPCR detection of miR-425 expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); B. RT-qPCR detection of CPEB1 mRNA expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); C-D. Protein bands and protein expression of CPEB1 by Western blot analysis (n = 93); E. Pearson correlation analysis of the relation between miR-425 expression and CPEB1 mRNA expression in lung cancer patients; F. miR-425 and CPEB1 mRNA expression in HFL1 and lung cancer cell lines (A549, NCI-H1299 and NCI-H292); G. Targetscan prediction of the targeting relationship between miR-425 and CPEB1; H. Dual luciferase reporter gene assay validation of the targeting relationship between miR-425 and CPEB1. Data in the figure were expressed by mean ± standard deviation; Comparison between two groups was analyzed by t-test.

    Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Luciferase, Reporter Gene Assay, Biomarker Discovery, Standard Deviation, Comparison



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    96
    ATCC normal human embryonic lung fibroblast hfl1
    MiR-425 is up-regulated and CPEB1 is down-regulated in lung cancer tissues; miR-425 targets CPEB1. A. RT-qPCR detection of miR-425 expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); B. RT-qPCR detection of CPEB1 mRNA expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); C-D. Protein bands and protein expression of CPEB1 by Western blot analysis (n = 93); E. Pearson correlation analysis of the relation between miR-425 expression and CPEB1 mRNA expression in lung cancer patients; F. miR-425 and CPEB1 mRNA expression in <t>HFL1</t> and lung cancer cell lines (A549, NCI-H1299 and NCI-H292); G. Targetscan prediction of the targeting relationship between miR-425 and CPEB1; H. Dual luciferase reporter gene assay validation of the targeting relationship between miR-425 and CPEB1. Data in the figure were expressed by mean ± standard deviation; Comparison between two groups was analyzed by t-test.
    Normal Human Embryonic Lung Fibroblast Hfl1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human embryonic lung fibroblast hfl1/product/ATCC
    Average 96 stars, based on 1 article reviews
    normal human embryonic lung fibroblast hfl1 - by Bioz Stars, 2026-06
    96/100 stars
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    MiR-425 is up-regulated and CPEB1 is down-regulated in lung cancer tissues; miR-425 targets CPEB1. A. RT-qPCR detection of miR-425 expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); B. RT-qPCR detection of CPEB1 mRNA expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); C-D. Protein bands and protein expression of CPEB1 by Western blot analysis (n = 93); E. Pearson correlation analysis of the relation between miR-425 expression and CPEB1 mRNA expression in lung cancer patients; F. miR-425 and CPEB1 mRNA expression in HFL1 and lung cancer cell lines (A549, NCI-H1299 and NCI-H292); G. Targetscan prediction of the targeting relationship between miR-425 and CPEB1; H. Dual luciferase reporter gene assay validation of the targeting relationship between miR-425 and CPEB1. Data in the figure were expressed by mean ± standard deviation; Comparison between two groups was analyzed by t-test.

    Journal: Regenerative Therapy

    Article Title: Human bone marrow mesenchymal stem cell-derived exosomes containing microRNA-425 promote migration, invasion and lung metastasis by down-regulating CPEB1

    doi: 10.1016/j.reth.2022.03.007

    Figure Lengend Snippet: MiR-425 is up-regulated and CPEB1 is down-regulated in lung cancer tissues; miR-425 targets CPEB1. A. RT-qPCR detection of miR-425 expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); B. RT-qPCR detection of CPEB1 mRNA expression in lung cancer tissues (n = 93) and adjacent normal tissues (n = 93); C-D. Protein bands and protein expression of CPEB1 by Western blot analysis (n = 93); E. Pearson correlation analysis of the relation between miR-425 expression and CPEB1 mRNA expression in lung cancer patients; F. miR-425 and CPEB1 mRNA expression in HFL1 and lung cancer cell lines (A549, NCI-H1299 and NCI-H292); G. Targetscan prediction of the targeting relationship between miR-425 and CPEB1; H. Dual luciferase reporter gene assay validation of the targeting relationship between miR-425 and CPEB1. Data in the figure were expressed by mean ± standard deviation; Comparison between two groups was analyzed by t-test.

    Article Snippet: Normal human embryonic lung fibroblast HFL1 and A549 lung cancer cells were purchased from Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China) while human lung cancer cells (NCI-H1299, NCI-H292) were from ATCC (VA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Luciferase, Reporter Gene Assay, Biomarker Discovery, Standard Deviation, Comparison